"Searching for Markers of Cancer using 2-D Chromatography", a free webcast by Dr. David M. Lubman, Professor of Chemistry University of Michigan - is now available on-demand. This webcast was first broadcasted live at the Research Lab Expo (RELEX), an online conference and virtual exhibition launched by the Reed Business Information Science
New York, NY (PRWEB) September 7, 2005 -- Searching for Markers of Cancer Using 2-D Chromatography", a free webcast by Dr. David M. Lubman, Professor of Chemistry University of Michigan - is now available on-demand.
This webcast was first broadcasted live at the Research Lab Expo (RELEX), an online conference and virtual exhibition launched by the Reed Business Information Science.
To register for this free webcast, visit: http://relex.unisfair.com/registration.jsp?code=1223
Webcast summary:
The protein expression of prostate and ovarian tumor cells are studied to search for potential markers of cancer. Fresh frozen tumor cells are obtained and prepared for study by the pathology groups at the University of Michigan Medical Center. The tumors are lysed and separated using a 2-D liquid separation, which is capable of separating out nearly 3000 protein bands. This method involves separation based upon pI in the first dimension using chromatofocusing and by hydrophobicity in the second dimension using nonporous silica (NPS) RP-HPLC.
The liquid eluent from the second dimension is then split and 1/3 is directed to an electrospray time-of-flight mass spectrometer (ESI-TOF MS) for mass analysis, while the remaining 2/3 is collected by a fraction collector for MALDI-TOF MS analysis. The result is a mass map as a function of pI analogous to 2-D gel electrophoresis. The ESI-TOF MS provides an accurate MW value, which can be used with MALDI-TOF MS peptide mapping and MS/MS for accurate identification of proteins in these human cancer cells. In addition, the MW value provides a means to determine whether a protein is modified including the presence of truncations, deletions, sequence changes and splice variants.
Using this method large numbers of tumor samples can be mapped and each type of cancer can be classified according to its protein profile. In addition, marker proteins have been identified which can be associated with different grades and stages of cancer progression. The method provides a means for pathologists to identify the grade of the tumor, i.e. whether it is more benign or malignant, and consequently determine a specific treatment for that tumor.
To register for this FREE webcast, visit: http://relex.unisfair.com/registration.jsp?code=1223
About Dr. David M. Lubman:
David M. Lubman received his Ph.D. in physical chemistry from Stanford University in 1979 under the direction of Professor Richard N. Zare. He was a fellow at the Weizmann Institute of Science and arrived at the University of Michigan in 1983 as a member of the Department of Chemistry.
He was promoted to Associate Professor in 1987 and Full Professor in 1991. Among a number of awards in the field, he has won an Alfred P. Sloan Foundation Award, the Meggars Award of the Society of Applied Spectroscopy, an R & D 100 Award and was recently elected as a Fellow of the American Association for the Advancement of Science. He has also won internal awards within the University of Michigan including the LS&A Excellence in Research Award, LS&A Excellence in Teaching Award and the Faculty Recognition Award.
His present research interests involve developing new proteomic methods for searching for protein markers of cancer. These include 2-D liquid mapping of tumor cells and serum and also the development of protein and aptamer microarray technology. Various collaborations with clinical researchers include studies in prostate, ovarian and breast cancer.
Dr. Lubman presently serves on the Editorial boards of J. Chromatography, Current Analytical Chemistry, Anal Chim Acta, and Mass Spectrometry Reviews. He is a member of American Association for the Advancement of Science, American Chemical Society and the American Society for Mass Spectrometry.
To register for this free webcast, visit: http://relex.unisfair.com/registration.jsp?code=1223
About RELEX:
The Research Lab Expo (RELEX) is an online conference and virtual exhibition launched by the Reed Business Information Science Group. The online event features a high quality educational forum comprised of multiple live free webcasts.
The Research Lab Expo offers cutting-edge compelling web content to industry professionals and decision makers across multiple industries, including aerospace and defense, automotive, chemicals, petroleum, coatings, inks, adhesives, textiles, computers, software, office equipment, energy, utilities, environmental, food, beverage, tobacco, government lab, industrial equipment, instruments, laboratory, design, manufacturing, metal, materials, pharmaceuticals, biopharmaceuticals, healthcare, plastic, rubber, paper, pulp wood, scientific, medical, test instruments, semiconductors, electronic components, and telecom.
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